Human Granulocytes Detected with a Piezoimmunosensor
作者:
Bernd König,
Michael Grätzel,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 11
页码: 2313-2328
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017472
出版商: Taylor & Francis Group
关键词: Piezoelectric Immunosensor;Biosensor;Human Granulocytes;Anti-CD 18 Antibody;Whole Blood
数据来源: Taylor
摘要:
We have developed a reusable piezoelectric immunosensor for human granulocytes. Three different methods for immobilization of the anti-CD 18 antibody on the gold electrode of the immunosensor were tested. The methods showed some differences in terms of stability and sensitivity. Applying an anti-CD 18 antibody layer onto a 10-MHz AT-cut crystal resulted for 2 × 103to 3 × 105cells on the surface in a linear frequency change. The modified crystal was stable for eight weeks when stored desiccated at room temperature.
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