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Lactose Determination in Raw Milk with a Two-Enzyme Based Electrochemical Sensor

 

作者: R. Pilloton,   M. Mascini,   I.G. Casella,   M.R. Festa,   E. Bottari,  

 

期刊: Analytical Letters  （Taylor Available online 1987）
卷期: Volume 20, issue 11  

页码: 1803-1814

 

ISSN:0003-2719

 

年代: 1987

 

DOI:10.1080/00032718708082597

 

出版商: Taylor & Francis Group

 

关键词: LACTOSE SENSOR;MILK;IMMOBILIZED ENZYMES;ELECTROCHEMICAL SENSORS

 

数据来源: Taylor

 

摘要:

A hydrogen peroxide electrochemical sensor, coupled with two enzymes (ß -galactosidase and glucose oxidase) immobilized on the surface of the sensor covered with a cellulose acetate dialysis membrane (cut off 100), is proposed for the analysis of lactose in raw milk. The resulting hydrogen peroxide formation is measured with a platinum electrode polarized at +650 mV versus Ag/AgCl. The procedure developed is very simple and the short response time permits its use in assaying milk samples on dairy farms.

 

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