A Nuclear Marker for Mammalian Cells and Its Use with Intracerebral Transplants
作者:
TourbahAyman,
GansmullerAnne,
GumpelMadeleine,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1991)
卷期:
Volume 66,
issue 1
页码: 29-34
ISSN:1052-0295
年代: 1991
DOI:10.3109/10520299109110546
出版商: Taylor&Francis
关键词: Hoechst staining;central nervous system;mammals;transplantations;immunohistochemistry;myelin
数据来源: Taylor
摘要:
The Hoechst dye staining method has been successfully applied to the central nervous system in mammals and its use has been demonstrated in intracerebral transplantation. The technique is rapid, simple and based on intrinsic nuclear properties. It was found to be permanent and valid whatever the animal strains or ages, allowing the distinction of rat cells from those of mouse, studied either separately or in a cross-transplantation model. It permitted the detection of grafted cells in the area of transplantation and the observation of early dispersion around the implantation site. Moreover, it can be combined with immunohistochemistry as demonstrated by a myelin marker in a relevant model. Immunodetection can thus help to directly observe grafted cells, at distance from the locus of transplantation, confirming their presence in the graft-type myelin patches.Because of its rapid performance, this technique can be used systematically after transplantation to check for the presence of grafted cells in the host.
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