Complete mutation detection using unlabeled chemical cleavage
作者:
Jennifer A. Saleeba,
Susan J. Ramus,
Richard G. H. Cotton,
期刊:
Human Mutation
(WILEY Available online 1992)
卷期:
Volume 1,
issue 1
页码: 63-69
ISSN:1059-7794
年代: 1992
DOI:10.1002/humu.1380010110
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: β‐thalassemia;Sickle cell anemia;Nonradioactive;DNA mutations;Silver stain
数据来源: WILEY
摘要:
AbstractWe have developed a strategy for the complete detection of point mutations, small insertions and deletions by chemical cleavage based on the methodology of Cotton et al. (1988). The technique was extended by the development of a nonisotopic cleavage product detection system using silver staining after gel electrophoresis. The complete mutation detection was achieved by use of mutant and wild‐type DNAs in equimolar quantities in duplex formation, thus any mismatches that are resistant to chemical cleavage (e.g., some T·G mismatches) are easily detected by cleavage of the complementary heteroduplex (e.g., A·C mismatch). With such a strategy mutant DNAs can be screened for mutations and polymorphisms. The advantages of complete unlabeled mutation detection are considerable. © 1992 Wiley‐Lis
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