α2-Adrenoceptor Activation Increases Calcium Channel Currents in Single Vascular Smooth Muscle Cells Isolated from Human Omental Resistance Arteries
作者:
A.D. Hughes,
N.A. Parkinson,
S. Wijetunge,
期刊:
Journal of Vascular Research
(Karger Available online 1996)
卷期:
Volume 33,
issue 1
页码: 25-31
ISSN:1018-1172
年代: 1996
DOI:10.1159/000159128
出版商: S. Karger AG
关键词: α2-Adrenoceptor;Calcium channel;G protein;Pertussis toxin;Vascular smooth muscle
数据来源: Karger
摘要:
Single cells were freshly isolated from human omental resistance arteries using an enzymatic dispersion technique. Calcium channel currents (IBa) were recorded using whole cell voltage clamp techniques with Ba2+ as the charge carrier. BHT 933, a selective α2-adrenoceptor agonist, increased IBa. The effect of BHT 933 was reversible following washout. The action of BHT 933 was blocked by yohimbine. Pretreatment of tissues with pertussis toxin for 18 h or inclusion of GDP-β-S in the intracellular patch pipette solution also prevented the BHT 933-induced rise in IBa, but had no effect on IBa in the absence of BHT 933. Activation of α2-adrenoceptors in human vascular smooth muscle cells increases IBa by a mechanism involving a pertussis toxin-sensitive G prote
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