An integrated set of methods for routine flow cytometric DNA analysis are described. The methods consist of procedures for sample acquisition, storage, standardization, staining and statistical analysis. Our experience with the methods over the past ten years is reviewed. It is concluded that only with adequate solutions to all methodological steps involved, is it possible to produce reliable end‐point results. Flow cytometric DNA analysis is a valuable research tool in clinical and experimental oncology. Final evaluation of the prognostic significance of DNA analysis in neoplastic disease awaits the results of ongoing studie