In this study,in vitroosteoblast responses to glow-discharged, commercially pure titanium (Ti) surfaces were investigated. It was hypothesized that the glow-discharge treatment would be an effective sterilization procedure for Ti implantations before implantation. The Ti surfaces were prepared by grinding to 600 grits followed by cleaning. These were then divided into two groups, with one group being the control and the other group undergoing glow-discharge treatment using oxygen. Human embryonic palatal mesenchyme cells, an osteoblast precursor, were used to evaluate the cell responses to glow-discharged and control Ti surfaces. It was observed from this study that protein production and osteocalcin production on both surfaces exhibited no significant differences during the 10-day study. Similarly, no significant differences were observed for alkaline phosphatase (ALP) specific activity during the first 7 days of incubation. However, at day 10, the ALP specific activity for control Ti surfaces was significantly higher than the ALP activity for the glow-discharged surface. Overall, this study suggested that the use of glow discharge as an alternative sterilization procedure for medical and dental implants did not inhibit osteoblast phenotypic expression.