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Enzymatic Flow Injection Determination of Gamma-Aminobutyric Acid

 

作者: H. Horie*,   G.A. Rechnitz,  

 

期刊: Analytical Letters  （Taylor Available online 1995）
卷期: Volume 28, issue 2  

页码: 259-266

 

ISSN:0003-2719

 

年代: 1995

 

DOI:10.1080/00032719508000320

 

出版商: Taylor & Francis Group

 

关键词: GABA;FIA;NADPH;GABAse;Fluorescence;Green tea

 

数据来源: Taylor

 

摘要:

γ-Aminobutyric acid (GABA) was determined by using flow injection analysis with a column of GABAse immobilized on glass beads. The NADPH produced was monitored fluorometrically. At a flow rate of 0.8 ml/min, peak heights were linear in the range 1-500 μM, and samples could be injected every minute. Nearly 100 % recovery of GABA was observed when GABA was added to infusions of green tea.

 

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