Individual and Simultaneous Stopped-Flow Fluorimetric Determination of Perphenazine and Chlorpromazine
作者:
M.C. Gutiérrez,
A. Gómez-Hens,
D. Pérez-Bendito,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 11
页码: 1847-1865
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708082600
出版商: Taylor & Francis Group
关键词: Stopped-flow;fluorimetry;perphenazine;chlorpromazine;mixtures;Pharmaceuticals
数据来源: Taylor
摘要:
The stopped-flow technique is used to develop fluorimetric determinative methods for two phenothiazine drugs: perphenazine and chlorpromazine. The methods are based on the oxidation of these compounds to their corresponding fluorescent sulfoxides. Owing to the intrinsic characteristics of this technique, which allow rapid and thorough mixing of reactants, the oxidation is affected by dissolved oxygen itself, which therefore makes an oxidizing reagent unnecessary. Two methods are proposed for each phenothiazine, one based on initial rate and another on maximum fluorescence intensity measurements. Their linear ranges are 0.1–40 μg mL−1and their precision (%RSD) between 0.9 and 2.8%. These methods have been satisfactorily applied to the determination of both phenothiazines in several pharmaceutical preparations. On the basis of the additivity of the initial rate and maximum fluorescence intensity of perphenazine and chlorpromazine, the proportional equations principle is applied to the simultaneous determination of these compounds. Perphenazine/chlorpromazine mixtures in ratios between 1:8 and 8:1 are successfully resolved.
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