Protein A immobilization and HIgG adsorption onto porous/nonporous and swellable HEMA-incorporated polyEGDMA microspheres
作者:
H. Ayhan,
K. Kesenci,
E. Pişkin,
期刊:
Journal of Biomaterials Science, Polymer Edition
(Taylor Available online 2000)
卷期:
Volume 11,
issue 1
页码: 13-25
ISSN:0920-5063
年代: 2000
DOI:10.1163/156856200743463
出版商: Taylor & Francis Group
关键词: POLY(EGDMA/HEMA) MICROBEADS;SWELLABLE/NON SWELLABLE;SURFACE MODIFICATION;PROTEIN A IMMOBILIZATION
数据来源: Taylor
摘要:
Both non swellable and swellable poly(EGDMA/HEMA) microbeads were produced by suspension copolymerization. These microbeads were modified by immobilization of a spacer-arm (hexamethylene diamine (HMDA)) and protein A. The optimal values for modifications were as follows: sodium periodate concentration, 1.0 mg ml-1; HMDA concentration, 4 mg ml-1; and glutaraldehyde concentration, 0.070μg ml-1. Adsorption of protein A onto the plain and periodate oxidized poly(EGDMA/HEMA) microbeads were very close to each other, and were 0.01-0.02 mg protein A on the 1-g Microbeads I and II, respectively. Protein A immobilization on poly(EGDMA/HEMA) microbeads were studied at different temperatures, times, and pHs using single protein solution containing different amounts of proteins. The optimal values for immobilization were as follows: the initial protein A concentration, 0.1 mg ml-1; temperature, 25°C; pH, 9.5; and immobilization time, 120 min. Incorporation of protein A resulted in 1.420 and 1.825 mg protein A on the 1-g Microbeads I and II, respectively. HIgG adsorption capacity on the protein A-incorporated poly(EGDMA/HEMA) microbeads is 27 and 35 mg HIgG g-1polymer for Microbeads I and II, respectively.
点击下载:
PDF (212KB)
返 回