Solid‐phase synthesis of chicken vasoactive intestinal peptide by a mild procedure usingNα‐9‐fluorenylmethyloxycarbonyl amino acids
作者:
ROBERTO COLOMBO,
期刊:
International Journal of Peptide and Protein Research
(WILEY Available online 1982)
卷期:
Volume 19,
issue 1
页码: 71-78
ISSN:0367-8377
年代: 1982
DOI:10.1111/j.1399-3011.1982.tb03025.x
出版商: Blackwell Publishing Ltd
关键词: p‐benzyloxybenzylamide resin anchorage;tert.‐butyl‐type side chain protection;Nα‐9‐fluorenylmethyloxycarbonylamino acids;nonhydrolytic base cleavage;trifluoroacetic acid cleavage of peptide amide from resin;chicken vasoactive intestinal peptide (VIP);soli
数据来源: WILEY
摘要:
The octacosapeptide amide corresponding to the entire amino acid sequence of chicken vasoactive peptide (VIP) was assembled on ap‐benzyloxybenzylamine resin support using the base‐labile 9‐fluorenylmethyloxycarbonyl asNα‐protecting group, cleaved by mild acid treatment, and purified by gel‐filtration and ion‐exchange chromatography. The symmetrical anhydride coupling was employed and monitored by two independent methods, and acetic anhydride termination was incorporated to minimize formation of deletion peptides. The homogeneity of the final product, obtained in 18% yield, was assessed by t.l.c., disc electrophoresis, amino‐terminal amino acid analysis, and amino acid analyses of acid and enzyme hydrolysates. The purified chicken VIP was shown to be active on gastric acid secretion and on pancreatic blood flow. Previously reported ring closure of the Asp‐Asn unit seemed to be at a minimum, owing to the mild basic and
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