Structural stabilization for electron microscopy of pollen from herbarium specimens
作者:
J.R. Rowley,
S. Nilsson,
期刊:
Grana
(Taylor Available online 1972)
卷期:
Volume 12,
issue 1
页码: 23-30
ISSN:0017-3134
年代: 1972
DOI:10.1080/00173137209427642
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
The rehydration of dried pollen and spores is a common technique to obtain information on nuclear condition, cytoplasmic storage materials, the intine and other aspects of pollen morphology when fresh material is unavailable. Pollen grains are rehydrated and consequently expanded by most aqueous solutions although electron microscopical examination will reveal considerable variation both in stainability and quality of expansion following embedding. That variation, we suggest, is due to substances extracted during hydration and processing. Rehydration of herbarium material with aldehyde solutions containing cations known to stabilize mucosubstances has greatly improved staining and recovery of shape equivalent to fresh pollen ofNymphoidesandMenyanthes(Menyanthaceae). It is our contention that rehydration of dry pollen with attention to the stabilization of mucopolysaccharides substantially increases the information available from herbarium material and should supplement KOH and acetolyzed specimens.
点击下载:
PDF (2883KB)
返 回