SummaryThe newborn beagle pup serves as a model for neonatal intraventricular hemorrhage (IVH). Fluctuations in germinal matrix blood flow are felt to play a major role in the pathogenesis of IVH. We studied regional cerebral blood flow in awake newborn beagle pups utilizing [14C]-iodoantipyrine as a blood flow indicator and quantitative autoradiography. The equilibrium [tissue]:[blood] partition coefficient for iodoantipyrine was 1.13 ± .06 for grey matter. Blood flow was calculated for cerebral cortex (frontal = 59 ± 9 ml/100 g/min), 14 subcortical nuclear structures (e.g., caudate = 45 ± 6 ml/100 g/min), 3 white matter structures (centrum semiovale = 7 ± 1 ml/100 g/min), and germinal matrix (7 ± 1 ml/100 g/min) (mean ± S.E.).We conclude that under normal physiologic conditions the germinal matrix receives relatively low blood flow. This information can be used for comparison with germinal matrix blood flow during adverse experimental conditions.