A comparative study on the sorption behaviour of tungsten and rhenium between anion exchange resin, charcoal and alumina and aqueous media of HNO3, HCl and NaCl were carried out. The equilibrium distribution coefficient obtained for the different systems were evaluated and discussed in terms of the separation factor between W and Re. It is found that the systems based on alumina and HCl or NaCl are the most promising systems to be used for development of188W-188Re generator. The capacity of a chromatographic column loaded with alumina for188W from 0.01 M HCl, 0.7% NaCl and 1.2% NaCl was found to equals 75.3, 91.0 and 126.0 mg/cm, respectively. A recommended procedure was adopted for188W-188Re generator.