Production and characterization of antibodies to mouse scrapie‐amyloid protein elicited by non‐carrier linked synthetic peptide immunogens
作者:
Alessandro Di Martino,
Emilio Bigon,
Giuseppe Corona,
Lanfranco Callegaro,
期刊:
Journal of Molecular Recognition
(WILEY Available online 1991)
卷期:
Volume 4,
issue 2‐3
页码: 85-91
ISSN:0952-3499
年代: 1991
DOI:10.1002/jmr.300040207
出版商: John Wiley&Sons, Ltd.
数据来源: WILEY
摘要:
AbstractTwo polyclonal antibodies were raised by immunizing rabbits with two non carrier‐linked synthetic peptides whose amino acid sequences corresponded to codons 89–107 (peptide P1) and 219–233 (peptide P2) of the translated cDNA sequence of murine PrP protein. These free peptides, whose structural characteristics in solution were studied by circular dichroism, elicited a reasonable immunologic response in animals. Both antibodies still recognized the corresponding immunogens after affinity chromatography purification. However, only antibodies raised to the former sequence reacted by immunobolt with a purified preparation of murine scrapie amyloid protein. These findings are discussed together with their correlation to peptide structure and the effectiveness of this simplified immunization proc
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