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A comparison of culture methods for tobacco (Nicotiana tabacum) anthers

 

作者: AnitaE. Steyn,   J.A. Brink,   L.A. Coetzer,  

 

期刊: South African Journal of Plant and Soil  （Taylor Available online 1997）
卷期: Volume 14, issue 1  

页码: 27-30

 

ISSN:0257-1862

 

年代: 1997

 

DOI:10.1080/02571862.1997.10635076

 

出版商: Taylor & Francis Group

 

关键词: Anther culture;double layer medium;liquid medium;Nicotiana tabacum;solidified medium

 

数据来源: Taylor

 

摘要:

Three different anther culture methods for the South African tobacco cultivars C11 × WOSB and CDL 28 were tested. The yield of haploid plantlets produced using a solidified or double-layer medium was significantly higher than with the use of a liquid culture medium. The former two media showed no significant differences. Important considerations which must be taken into account when deciding on the optimum culture method for anther cultures are also discussed.

 

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