A Comparison of Substrates for Human Umbilical Vein Endothelial Cell Culture
作者:
SmeetsEdgar F.,
von AsmuthEckhardt J. U.,
van der LindenCees J.,
LeeuwenbergJet F. M.,
BuurmanWim A.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 4
页码: 241-250
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110072
出版商: Taylor&Francis
关键词: human endothelial cells;culture substrates;gelatin;fibronectin;silver staining;PECAM-1 (CD31) expression
数据来源: Taylor
摘要:
We have studied culture conditions which facilitate the growth of stable, non-proliferating, human umbilical vein endothelial cell (HUVEC) monolayers. Gelatin and fibronectin coatings, with or without glutaraldehyde cross-linking, on both plastic and glass were investigated for initial attachment of HUVEC and growth characteristics. The presence during culture of intercellular (IC) junctions demonstrated by silver staining, expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) and maintenance of a cobblestone appearance of HUVEC monolayers were assessed over time.Glutaraldehyde cross-linked fibronectin and gelatin coatings on glass and glutaraldehyde cross-linked gelatin or untreated fibronectin coatings on plastic served as good substrates for short term culture. Long term (20 days) cultures of HUVEC which maintained silver and PECAM-1 staining of IC junctions and a cobblestone appearance could be achieved if glutaraldehyde cross-linked gelatin coatings on glass were used as substrates.
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