Detection of Coxsackie B4 Virus RNA in Infected Mouse Kidneys by in situ Hybridization
作者:
Shigeo Mutoh,
Kazuo Kumada,
Kazunari Kume,
Junzou Suzuki,
Tomoyuki Yokota,
Hitoshi Suzuki,
期刊:
Nephron
(Karger Available online 1994)
卷期:
Volume 67,
issue 3
页码: 340-345
ISSN:1660-8151
年代: 1994
DOI:10.1159/000187990
出版商: S. Karger AG
关键词: Experimental glomerulonephritis;Coxsackie B4 virus;Hybridization;in situ;Nonradioactive oligonucleotide probe
数据来源: Karger
摘要:
We describe a method for detecting virus-specific RNA sequences using a nonradioactive enzyme system, and analyze coxsackie B4 virus (cox. B4) RNA sequences in infected mouse kidney with pathological changes. Diffuse mesangial proliferation was observed transiently 24 h after inoculation of cox. B4 virus in glomeruli. OCT-treated thin sections of the same tissue were hybridized in situ using a digoxigenin-labeled oligonucleotide probe. The strongest signal was observed in the mesangial sample. These findings suggest that the transient proliferative mesangial changes were caused by direct injury following virus infection without immune complex.
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