Utilization of Polyspecific Antiserum for Specific Radioimmunoassays: Radioimmunoassays for Rat Fetuin and Bikunin Were Developed by Using Antiserum Against Total Rat Serum Proteins
作者:
Edwin Fink,
Gudrun Godec,
Marianne Arnhold,
Peter Nawratil,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 12
页码: 2587-2594
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017976
出版商: Taylor & Francis Group
关键词: Radioimmunoassay;polyspecific antiserum;rat fetuin;rat bikunin
数据来源: Taylor
摘要:
Polyspecific antiserum against total rat serum proteins was used to develop specific and sensitive radioimmunoassays for fetuin and bikunin, two minor protein components of rat plasma. The radioimmunoassays proved to be highly useful to trace bikunin and fetuin in the course of developing isolation procedures, since neither specific functional assays nor monospecific antisera were available. The two examples demonstrate that, in general, it will be possible to develop a specific and sensitive radioimmunoassay with antiserum raised against a crude antigen preparation, such as a body fluid or a tissue extract, provided that a minute amount of pure antigen is available for preparing the radioiodinated antigen.
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