The Electrochemical Activity Determination of γ-Glutamyl Transpeptidase
作者:
J.M. Nigretto,
期刊:
Analytical Letters
(Taylor Available online 1984)
卷期:
Volume 17,
issue 16
页码: 1863-1876
ISSN:0003-2719
年代: 1984
DOI:10.1080/00032718408077189
出版商: Taylor & Francis Group
关键词: γ glutamyl transpeptidase;electrogenic substrates;electrochemistry
数据来源: Taylor
摘要:
The electrochemical activity determination of γ-glutamyl-transpeptidase is described using the electrogenic L-γ-glutamyl paraaminodiphenylamide substrate. In order to correlate the results with classical measurements involving chromogenic substrates, linear calibration curves for the amperometric detection of the released free amine, the kinetics of the catalysis and the enzyme level in one plasma sample have been compared to photometric experiments effectuated in parallel runs, in the same conditions, with L-y-glutamyl paranitroanilide as the substrate. Results show that this new technique applies successfully to such evaluations.
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