Use of isoelectric focusing to define major histocompatibility complex class I polymorphism in goats
作者:
I Joosten,
M F Sanders,
E J Hensen,
G Ruff,
期刊:
Animal Genetics
(WILEY Available online 1993)
卷期:
Volume 24,
issue 1
页码: 47-51
ISSN:0268-9146
年代: 1993
DOI:10.1111/j.1365-2052.1993.tb00918.x
出版商: Blackwell Publishing Ltd
关键词: MHC class I;goat;isoelectric focusing;caprine leucocyte antigen;CLA
数据来源: WILEY
摘要:
SummaryWe have used biochemical methods to extend and improve serological class I typing using a panel of 77 Swiss goats of the Saanen breed, comprising dam‐offspring combinations from six half‐sib sire families and several unrelated animals. Of these animals class I molecules were precipitated from cell lysates with the mAb B1.1G6 and HC10. Immunoprecipitates were analysed by SDS‐PAGE and 1D‐IEF. There was a good agreement between class I serological types and IEF banding patterns. We have identified three new class I specificities and subdivided the Bel 7 specificity. IEF has enabled us to make planned immunizations to produce antisera to the new specificities. New evidence for the expression of a second class I locus product in the Be7 haplotype has bee
点击下载:
PDF
(703KB)
返 回