Field tests conducted in a relict prairie using sticky traps baited with a broad range of adult diabroticine attractants showedDiabrotica cristata(Harris) shares some chemosensory responses with bothD. barberiSmith and Lawrence andD. virgifera virgiferaLeConte.D. cristatawas attracted to estragole, β-ionone,para-methoxycinnamaldehyde, and a mixture of trimethoxybenzene, indole, and cinnamaldehyde (=TIC mixture) (attractants for adults ofD. virgifera virgiferain corn tests), as well as eugenol, isoeugenol, and cinnamyl alcohol (attractants for adults ofD. barheriin corn tests). Several of the active compounds, such as cinnamyl alcohol and β-ionone, are structurally distinct from the previously described eugenol-type attractants forD. barberiandD. cristataand represent new semiochemicals for the genus. In the prairie trials,D. barberiadults were anomalously attracted to two lures (estragole and the TIC mixture) that were not active in corn evaluations.