Short Communication: Nuclear matrix proteins of bovine corneal and conjunctival epithelium
作者:
VassalloJohn C.,
KurpakusMichelle A.,
期刊:
Current Eye Research
(Taylor Available online 1996)
卷期:
Volume 15,
issue 8
页码: 899-904
ISSN:0271-3683
年代: 1996
DOI:10.3109/02713689609017632
出版商: Taylor&Francis
关键词: conjunctival epithelium;corneal epithelium;keratin;lamins;nuclear matrix;bovine (cattle)
数据来源: Taylor
摘要:
PurposeTo present a preliminary biochemical and immunochemical analysis of nuclear matrix proteins isolated from ocular surface epithelium.MethodsNuclear matrix protein-enriched fractions were prepared from bovine corneal and conjunctival epithelial cells. The preparations were analyzed by 1D and 2D SDS-PAGE and Western immunoblotting.ResultsA comparison of corneal and conjunctival nuclear matrix preparations using 1D and 2D SDS-PAGE revealed subsets of both common and apparently unique proteins. Western immunoblotting analysis of corneal nuclear matrix preparations with antibody to nuclear lamins confirmed the presence of these proteins in the preparation. 1D and 2D immunoblotting analysis of corneal nuclear matrix preparations with antibodies to the keratin K12 revealed the presence of two protein species.ConclusionsPreliminary biochemical analysis of ocular surface nuclear matrix provides evidence for cell-type specific components of this structure. Immunochemical analysis of corneal epithelial nuclear matrix preparations suggests that two keratin K12 pools may exist in these cells, one pool associated with the cytoplasmic intermediate filament network, and a second pool closely associated with the nuclear matrix framework. Keratin K12 may therefore play a role in the regulation of corneal epithelial cell gene and protein expression via its association with the nuclear matrix. Curr. Eye Res. 15: 899–904, 1996.
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