Conditioned Medium from Mouse Sarcoma 180 Cells Contains Vascular Endothelial Growth Factor
作者:
RosenthalRosalind A.,
MegyesiJoseph F.,
HenzelWilliam J.,
FerraraNapoleone,
FolkmanJudah,
期刊:
Growth Factors
(Taylor Available online 1990)
卷期:
Volume 4,
issue 1
页码: 53-59
ISSN:0897-7194
年代: 1990
DOI:10.3109/08977199009011010
出版商: Taylor&Francis
关键词: Vascular endothelial growth factor (VEGF);vascular permeability factor (VPF);mouse sarcoma 180 cells;endothelial cells;angiogenesis
数据来源: Taylor
摘要:
AbstractMedium conditioned by mouse sarcoma 180 cells stimulates the growth of capillary endothelial cells. The growth factor produced by mouse sarcoma 180 cells is heparin-binding, dithiothreitol-sensitive, endothelial cell specific, and secreted into the medium. The characteristics of this mouse sarcoma-derived growth factor are very similar to those of vascular endothelial growth factor (VEGF) first described by Ferrara and Henzel (1989). The N-terminal amino acid sequences of the two growth factors are similar. Since the amino acid sequence of vascular permeability factor (VPF) is essentially identical to that of VEGF, a Western blot of mouse sarcoma 180-derived endothelial growth factor was probed with a polyclonal antibody raised against human VPF. This antibody reacted with several proteins of approximately 23 kDa, suggesting the presence of multiple forms of a VEGF-like protein. A full length cDNA probe for bovine VEGF reacted strongly with RNA isolated from mouse sarcoma 180 cells. We conclude that an endothelial growth factor found in conditioned medium from mouse sarcoma 180 cells is VEGF.
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