Determination of Metoprolol Tartrate as Cu(II)-Dithiocarbamate Complex
作者:
Güzin Alpdoğan,
Sidika Sungur,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 7
页码: 1359-1368
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708007222
出版商: Taylor & Francis Group
关键词: Metoprolol tartrate;Cu (II) - Dithiocarbamate complex;high pressure liquid chromatography;tablets
数据来源: Taylor
摘要:
An HPLC method has been developed for the determination of the beta adrenergic blocking drug metoprolol tartrate. The method is based on the formation of a Cu (II) - dithiocarbamate complex by precolumn derivatization of secondary amino group of metoprolol with CS2and CuCl2in the presence of ammonia. The complex is extracted into chloroform and injected into the chromatograph. Chromatographic separation was performed by a RP (μ Bondapak C18) column with methanol - pH 5.8 phosphate buffer (80 : 20) as the isocratic mobile phase. A variable wavelength absorbance detector (λ = 275 nm) was used. A linear relationship was obtained between peak areas and metoprolol tartrate concentrations over the range of 35 - 180 nmol ml−1. The method was applied to the analysis of metoprolol tartrate tablets and the results were statistically compared with those obtained by official method using t - and F - tests.
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