&NA;Photodynamic therapy is under intense investigation as an adjuvant treatment for malignant glial tumors of the central nervous system. Photofrin‐II (HpD‐II) is currently the most actively investigated photosensitizing agent. A crucial issue regarding the safe and efficacious usage of HpD‐II‐based photodynamic therapy is the individual in vivo kinetics of tumor uptake and retention, compared with normal brain clearance. The optimal time for photoactivation of sensitized tumor must be known to ensure a high target‐to‐nontarget ratio, resulting in the maximal tumor destruction while preserving normal brain. Our laboratory developed a radionuclide scan based on111indium (111In)‐labeled HpD‐II to evaluate HpD‐II localization and clearance noninvasively within a canine model of intracerebral gliosarcoma. Synthesis of the111In‐HpD‐II complex in >90% yield is achieved by a simple, rapid labeling method. Radiochemical purity and stability were verified by high‐performance liquid chromatography. Using the canine model of intracerebral gliosarcoma, we followed the uptake of111In‐HpD‐II in tumors with serial scintillation scanning. Localization of the tumor by111In‐HpD‐II has been verified by contrast‐enhanced computed tomographic scan followed by gross and histological examination of the enhancing brain region. Total body biodistribution of111In‐HpD‐II at various times after injection has been evaluated. The ratio of uptake in tumor compared with surrounding brain peaked at 72 hours after injection. The knowledge of regional distribution and concentration of a photosensitizing agent within a tumor mass and surrounding brain allows for the most efficacious timing and localization of a photoactivating source. (Neurosurgery24:547‐556, 1989)