Optimisation of transient transformation of a South African spring wheat cultivar with particle bombardment
作者:
S.M. de Villiers,
Y. Laib,
期刊:
South African Journal of Plant and Soil
(Taylor Available online 2000)
卷期:
Volume 17,
issue 1
页码: 50-53
ISSN:0257-1862
年代: 2000
DOI:10.1080/02571862.2000.10634865
出版商: Taylor & Francis Group
关键词: β-giucuronidase;particle inflow gun;Triticum aestivum L.
数据来源: Taylor
摘要:
Transient transformation was optimised for bread wheat,Triticum aestivum L.cv Kariega, using a particle inflow gun constructed at ARC-Roodeplaat. Excised immature embryos, culturedin vitrofor 5 to 7 days, were bombarded with pAHC25 and analysed for the presence of the β-glucuronidase reporter gene. Three bombardment variables were optimised and most blue spots were obtained with a helium pressure of 600 kPa, 20 mm particle flight distance and 0.4 Mosmoticum in the bombardment medium. Furthermore, the maize ubiquitin promoter was shown to be functional in Kariega.
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