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Optimisation of transient transformation of a South African spring wheat cultivar with particle bombardment

 

作者: S.M. de Villiers,   Y. Laib,  

 

期刊: South African Journal of Plant and Soil  (Taylor Available online 2000)
卷期: Volume 17, issue 1  

页码: 50-53

 

ISSN:0257-1862

 

年代: 2000

 

DOI:10.1080/02571862.2000.10634865

 

出版商: Taylor & Francis Group

 

关键词: β-giucuronidase;particle inflow gun;Triticum aestivum L.

 

数据来源: Taylor

 

摘要:

Transient transformation was optimised for bread wheat,Triticum aestivum L.cv Kariega, using a particle inflow gun constructed at ARC-Roodeplaat. Excised immature embryos, culturedin vitrofor 5 to 7 days, were bombarded with pAHC25 and analysed for the presence of the β-glucuronidase reporter gene. Three bombardment variables were optimised and most blue spots were obtained with a helium pressure of 600 kPa, 20 mm particle flight distance and 0.4 Mosmoticum in the bombardment medium. Furthermore, the maize ubiquitin promoter was shown to be functional in Kariega.

 

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