Selectivity tuning of serially coupled columns in high performance liquid chromatography
作者:
Thomas Welsch,
Uwe Dornberger,
Dietmar Lerche,
期刊:
Journal of High Resolution Chromatography
(WILEY Available online 1993)
卷期:
Volume 16,
issue 1
页码: 18-26
ISSN:0935-6304
年代: 1993
DOI:10.1002/jhrc.1240160104
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Column liquid Chromatography;Serially coupled columns;Selectivity tuning by changing the flow rate;Stationary phases of different selectivity
数据来源: WILEY
摘要:
AbstractThe tuning of selectivity by changing the flow rate has been investigated in HPLC: two columns with different retention characteristics were coupled in seriesviaa T‐piece and the relative retention of components chromatographed on the system were changed by varying the individual flow rates in the coupled columns. The flow rate alteration was performed by adding a second flow after the first column. The flow rate ratio necessary for optimum resolution can be easily calculated on the basis of the capacity factors measured on the individual columns. The performance of this method for adjusting selectivity has been demonstrated by using different column combinations to separate several mixtures containing chlorophenols, nitroaromatic compounds, and aromatic hydrocarbon
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