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Partial Purification of Intracellular Murine Sarcoma-Leukemia Virus RNA Species by Membrane Filtration

 

作者: Nobuo Tsuchida,   Saumya Bhaduri,   Heschel J. Raskas,   Maurice Green,  

 

期刊: Intervirology  （Karger Available online 1973）
卷期: Volume 1, issue 1  

页码: 27-33

 

ISSN:0300-5526

 

年代: 1973

 

DOI:10.1159/000148829

 

出版商: S. Karger AG

 

关键词: Virus;RNA;Tumor;Sarcoma;Leukemia Poly(A)

 

数据来源: Karger

 

摘要:

Both 35S and 20S viral RNA species from transformed rat cells which produce the murine sarcoma-leukemia virus complex were retained on nitrocellulose membrane filters, yielding a 33-fold purification and over a 50 % recovery of virus-specific RNA. These findings suggest that intracellular 35S and 20S RNA species contain poly(A) tracts.

 

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