首页   按字顺浏览 期刊浏览 卷期浏览 Comparison of ELISA and HPLC techniques for the analysis of carbendazim and thiabendazo...
Comparison of ELISA and HPLC techniques for the analysis of carbendazim and thiabendazole residues in fruit and vegetables

 

作者: KatrinaA. Mountfort,   StewartL. Reynolds,   StephenA. Thorpe,   ShaunN. White,  

 

期刊: Food and Agricultural Immunology  (Taylor Available online 1994)
卷期: Volume 6, issue 1  

页码: 17-22

 

ISSN:0954-0105

 

年代: 1994

 

DOI:10.1080/09540109409354808

 

出版商: Taylor & Francis Group

 

关键词: ELISA;HPLC;carbendazim;thiabendazole

 

数据来源: Taylor

 

摘要:

An enzyme‐linked immunosorbent assay (ELISA) was compared with a high‐pressure liquid chromatography (HPLC) procedure for the analysis of carbendazim and thiabendazole in fruit and vegetables. The method was found to be rapid, simple and repeatable; the main disadvantages being that the ELISA used could not distinguish between carbendazim and thiabendazole and that there were matrix effects from solvents and sample extracts. Consequently, a simple screening procedure was devised for carbendazim in fruit and vegetables. Samples were extracted by maceration with methanol and analyzed using immunoassay kits. Recoveries from 100 to 123% were achieved. The screening procedure was applied to samples of fruit and vegetables over a period of 12 months and compared with conventional HPLC methodology. The correlation coefficient between the two techniques was 0.91 and the ELISA method represented a saving of 54% in time and used only 12% of the solvent used for HPLC analysis.

 

点击下载:  PDF (323KB)



返 回