Continuous enzyme processing: The isolation of prolyl‐tRNA synthetase fromPhaseolus Aureus
作者:
P. Dunnill,
J. A. Currie,
M. D. Lilly,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1970)
卷期:
Volume 12,
issue 1
页码: 63-74
ISSN:0006-3592
年代: 1970
DOI:10.1002/bit.260120106
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA pilot‐plant process has been developed for the continuous extraction and partial purification of prolyl‐tRNA synthetase from mung bean. The bean slurry was wet ground in a hammer mill, clarified by two‐stage centrifugation, and the protein in the effluent fractionated by precipitation at pH values of 5.2 and 4.2. The throughput was 13 kg dry bean/hr. The improved extraction process and reduced processing time resulted in an enzyme product with a specific activity 16 × that previously obtained in the batch process. The yield was also 50–60 time
点击下载:
PDF
(545KB)
返 回