This paper describes a method for quantifying oxalate in soil HC1 extracts using reversed‐phase ion‐pairing high performance liquid chromatography with UV detection at 220 nm. The method was adapted from a procedure for determining urinary oxalate (6). The mobile phase was 10 percent KH2PO4and 5 mM TBA adjusted to pH 2.0 with H3PO4. The analytical column was a totally porous, reversed‐phase silica based C‐8 column (Hibar Li‐Chrosorb™). An important step in this method was the pre‐ treatment of each soil extract with a reversed‐phase C‐18 column (SPICE™ C‐18). Sample pre‐treatment removed complex, non‐polar and low polarity compounds often present in soil extracts.