QUANTITATIVE COMPOSITION OF HIGH PALMITIC AND STEARIC ACID SOYBEAN OIL TRIACYLGLYCEROLS BY REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY: UTILIZATION OF EVAPORATIVE LIGHT SCATTERING AND FLAME IONIZATION DETECTORS
作者:
W.E. Neff,
G.R. List,
W.C. Byrdwell,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1999)
卷期:
Volume 22,
issue 11
页码: 1649-1662
ISSN:1082-6076
年代: 1999
DOI:10.1081/JLC-100101758
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Triacylglycerol species (TAG) of oils from five genetically modified soybean varieties were resolved by gradient reversed phase high performance liquid chromatography. The eluting TAG were detected and quantitated without the requirement for TAG response factors or calibration curves for the detector response by an improved evaporative light scattering detector and by a flame ionization detector. The accuracy of the improved light scattering detector for TAG analysis was checked by comparing the absolute error and average absolute error between experimental fatty acid composition obtained by calibrated gas chromatography of the transmethylated soybean oil and the calculated fatty acid composition obtained from the TAG composition of the soybean oil.
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