Applications of DAPI Cytochemistry to Neurobiology
作者:
SannaPietro Paolo,
JirikowskiGustav F.,
LewandowskiGail A.,
BloomFloyd E.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 6
页码: 346-350
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110047
出版商: Taylor&Francis
关键词: DAPI;cytochemistry;nervous system;immunofluorescence
数据来源: Taylor
摘要:
4′, 6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained usingβ-galactosidase. DAPI also allows easy identification of mitotic figures and can be used to supplement cytochemical studies involving cell division in the nervous system.
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