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Applications of DAPI Cytochemistry to Neurobiology

 

作者: SannaPietro Paolo,   JirikowskiGustav F.,   LewandowskiGail A.,   BloomFloyd E.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1992)
卷期: Volume 67, issue 6  

页码: 346-350

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110047

 

出版商: Taylor&Francis

 

关键词: DAPI;cytochemistry;nervous system;immunofluorescence

 

数据来源: Taylor

 

摘要:

4′, 6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained usingβ-galactosidase. DAPI also allows easy identification of mitotic figures and can be used to supplement cytochemical studies involving cell division in the nervous system.

 

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