Three Variants of Parathyroid Hormone-Related Protein Messenger RNA Are Expressed in Human Mammary Gland
作者:
ANDERSSON Y.,
LINDQUIST S.,
BERGSTRÖM S.,
HERNELL O.,
期刊:
Pediatric Research
(OVID Available online 1997)
卷期:
Volume 41,
issue 3
页码: 380-383
ISSN:0031-3998
年代: 1997
出版商: OVID
数据来源: OVID
摘要:
PTH-related protein (PTHrP) is found in a variety of tissues; particularly high levels are present in human milk. The structure of the human PTHrP gene is complex, and alternative splicing allows expression of three different variants PTHrP139, PTHrP173, and PTHrP141, respectively. To determine which of the variants are expressed in human mammary gland a reverse transcriptase polymerase chain reaction (RT-PCR) method was elaborated, distinguishing the three variants. mRNA isolated from human milk cells, human mammary epithelial cells (HMEC) and human nonlactating mammary gland cells were analyzed. The RT-PCR experiments resulted in amplification of DNA fragments corresponding to all three variants for all three cell sources tested. The nucleotide sequences of the PCR fragments were determined and verified to be identical to the reported sequences. Hence, it is concluded that human mammary gland epithelial cells express three variants of PTHrP. Whether these have different physiologic effects in the mammary gland or in the breast fed infant remain to be explored.Abbreviations: PTHrP,PTH-related protein;PCR,polymerase chain reaction;HMEC,human mammary epithelial cells
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