An Evaluation of the Gaspak System in the Culture of Anaerobic Bacteria
作者:
J. G. Collee,
B. Watt,
Elizabeth B. Fowler,
R. Brown,
期刊:
Journal of Applied Bacteriology
(WILEY Available online 1972)
卷期:
Volume 35,
issue 1
页码: 71-82
ISSN:0021-8847
年代: 1972
DOI:10.1111/j.1365-2672.1972.tb03675.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
Summary:Comparable results were obtained with a commercially available disposable system for the production of H2and CO2in an anaerobic jar (the Gaspak system) and with a standard anaerobic jar procedure. Both systems were used with the Baird&Tatlock anaerobic jar and a room temperature catalyst. The test organisms included strict anaerobes such asClostridium oedematienstype D andCl. tetani.Comparative tests were also made withBacteroidesspecies from laboratory stock cultures and with freshly isolated strains ofBacteroides.On occasion, higher recoveries were obtained with the standard system when relatively demanding models were set up but it has limitations, having been developed initially for research purposes. The Gaspak system probably gives more reliable and more reproducible results in a laboratory in which experience with strict anaerobes is limited or sporadic.
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