Scanning tunneling microscopy of DNA: Atom‐resolved imaging, general observations and possible contrast mechanism
作者:
M. G. Youngquist,
R. J. Driscoll,
T. R. Coley,
W. A. Goddard,
J. D. Baldeschwieler,
期刊:
Journal of Vacuum Science&Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena
(AIP Available online 1991)
卷期:
Volume 9,
issue 2
页码: 1304-1308
ISSN:1071-1023
年代: 1991
DOI:10.1116/1.585226
出版商: American Vacuum Society
关键词: DNA;GRAPHITE;SUBSTRATES;IMAGES;HIGH−RESOLUTION METHODS;SCANNING ELECTRON MICROSCOPY;ULTRAHIGH VACUUM;WAVE FUNCTIONS;MATHEMATICAL MODELS
数据来源: AIP
摘要:
We have shown that it is possible to image DNA with atomic resolution using scanning tunneling microscopy (STM), [R. J. Driscoll, M. G. Youngquist, and J. D. Baldeschwieler, Nature346, 294 (1990)]. Here we describe that data together with our general observations on STM of DNA in ultrahigh vacuum. We also suggest a possible contrast mechanism for DNA imaging by STM based on wave function orthogonality requirements between a molecule and its substrate. Topographic images are presented which resolve atomic features in addition to the double helical structure and nucleotide pairs of the DNA molecule. Comparisons of experimental STM profiles and modeled contours of the van der Waals surface ofA‐DNA show excellent correlation. Successive scans show that the imaging is nondestructive and reproducible. For this study, double‐stranded DNA was deposited on highly oriented pyrolitic graphite without coating, shadowing, or chemical modification.
点击下载:
PDF
(660KB)
返 回