Methyl Methacrylate Preparations of Canine Fetal Eye for Light Microscopy Evaluation
作者:
FischerMary W.,
期刊:
Journal of Histotechnology
(Taylor Available online 1985)
卷期:
Volume 8,
issue 4
页码: 197-199
ISSN:0147-8885
年代: 1985
DOI:10.1179/his.1985.8.4.197
出版商: Taylor&Francis
关键词: Canine fetal eye;methyl methacrylate;eye preparations
数据来源: Taylor
摘要:
AbstractThe meridional portion of a fetal canid eye at gestation day 55 was embedded in methyl methacrylate. Full sagittal sections, from 1 to 4 microns, passing through pupil and optic papilla or through peripapillary zones of dysplastic retina, were obtained using a Leitz base-sledge microtome with a profile d knife.Sections were floated onto glass slides with 50% ethanol, flattened with 95% ethanol, and dried on a 45' C warming plate. Plastic was removed with Cellosolve acetate, permitting a variety of routine and histochemical staining procedures.
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