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Methyl Methacrylate Preparations of Canine Fetal Eye for Light Microscopy Evaluation

 

作者: FischerMary W.,  

 

期刊: Journal of Histotechnology  (Taylor Available online 1985)
卷期: Volume 8, issue 4  

页码: 197-199

 

ISSN:0147-8885

 

年代: 1985

 

DOI:10.1179/his.1985.8.4.197

 

出版商: Taylor&Francis

 

关键词: Canine fetal eye;methyl methacrylate;eye preparations

 

数据来源: Taylor

 

摘要:

AbstractThe meridional portion of a fetal canid eye at gestation day 55 was embedded in methyl methacrylate. Full sagittal sections, from 1 to 4 microns, passing through pupil and optic papilla or through peripapillary zones of dysplastic retina, were obtained using a Leitz base-sledge microtome with a profile d knife.Sections were floated onto glass slides with 50% ethanol, flattened with 95% ethanol, and dried on a 45' C warming plate. Plastic was removed with Cellosolve acetate, permitting a variety of routine and histochemical staining procedures.

 

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