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STRATEGIES TO IMPROVE THE COLLOIDAL STABILITY AND THE REACTIVITY OF IMMUNOLATEX BEADS

 

作者: I. Miraballes-Martínez,   A. Martín-Rodríguez,   R. Hidalgo-Álvarez,  

 

期刊: Journal of Dispersion Science and Technology  (Taylor Available online 1996)
卷期: Volume 17, issue 3  

页码: 321-337

 

ISSN:0193-2691

 

年代: 1996

 

DOI:10.1080/01932699608943504

 

出版商: Taylor & Francis Group

 

关键词: Latex aggregation immunoassays;Sequential adsorption of proteins;Competitive adsorption of proteins;Polymer carriers

 

数据来源: Taylor

 

摘要:

The aim of the present work is to study the influence of the sequential or competitive adsorption of anti-HSA (Human Serum Albumin) IgG and RSA (Rabbit Serum Albumin) onto a negatively charged polystyrene latex on the colloidal stability of the immunoiatex (IgG-RSA/latex). Anionic (1-2) latex was prepared by surfactant free emulsion polymerization. Adsorption of IgG and RSA on the latex 1-2 was performed at low ionic strength and different pHs. Sequential and competitive adsorption of a-HSA IgG and RSA were performed

 

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