Many endocrine disturbances play an important role in the development of certain instances of obesity. Thyroidpituitary axis (prolactin) was studied in 91 individuals (37 diabetic obese, 25 non-diabetic obese and 29 control) from the out-patient clinic of Ismailia General Hospital in an attempt to find out a relation between prolactin and thermogenesis (thyroid hormones). The results obtained from the anthropometric measurements showed that there is a significant difference in the level of body fat percentage and body mass index when compared with those of the control croup. This was carried out to assure the state of obesity. The results also showed that although prolactin levels were significantly lower in the diabetic obese group than those of the obese non-diabetic and the control group there was a significant increase in the levels of T3in both diabetic and non-diabetic subjects when compared with those of the control group, while no significant difference was obtained in diabetic and non-diabetic obese groups when compared with those of the control group in the level of T3, T4, rT3and TSH.