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High performance liquid chromatography of spermidine and spermine using a postcolumn reactor of immobilized polyamine oxidase (Aspergillus terreus) Followed by Electrochemical Detection

 

作者: Noriyuki Watanabe,  

 

期刊: Biomedical Chromatography  （WILEY Available online 1992）
卷期: Volume 6, issue 1  

页码: 1-3

 

ISSN:0269-3879

 

年代: 1992

 

DOI:10.1002/bmc.1130060102

 

出版商: John Wiley&Sons, Ltd.

 

数据来源: WILEY

 

摘要:

AbstractA highly sensitive and selective method for the determination of spermidine and spermine has been developed. A polyamine oxidase (Aspergillus terreus) immobilized column was used as a postcolumn reactor. The detection limit was 0.2 pmol/injection for both spermidine and spermine with a linear range of three orders of magnitude.

 

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