Studies of the spore walls ofAgaricus bisporusandAgaricus campestris
作者:
C. Garcia Mendoza,
J. A. Leal,
M. Novaes-Ledieu,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1979)
卷期:
Volume 25,
issue 1
页码: 32-39
ISSN:0008-4166
年代: 1979
DOI:10.1139/m79-005
出版商: NRC Research Press
数据来源: NRC
摘要:
The composition and ultrastructure of spore walls ofAgaricus bisporusandAgaricus campestriswere compared by chemical and enzymatic assays and electron microscopy. Polymers ofN-acetylglucosamine (chitin) and glucosamine (chitosan) were the major carbohydrate wall components of both species. The chitin to chitosan ratio in the spore walls ofA.bisporuswas about 0.38, while inA.campestrisit was about 2.8. These polymers were associated with proteins, lipids (readily extractable and bound), melanin, and a low content of β-glucan. The quantity of protein, total lipid, melanin, or β-glucan was similar in spore walls of both species. With the electron microscope, the spore walls appeared as two well-defined layers which may correspond to microfibrils of chitin and chitosan in which the electron-dense melanin was located in the external layer.
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