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Staining Etched Epoxy Resin Sections for Light Microscopy

 

作者: IwadareTsukasa,   AraiTaizo,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1995)
卷期: Volume 70, issue 2  

页码: 53-56

 

ISSN:1052-0295

 

年代: 1995

 

DOI:10.3109/10520299509108317

 

出版商: Taylor&Francis

 

关键词: azan;2-bromoethylamine;epoxy section;ferrocene;hematoxylin-eosin;kidney biopsy;light microscopy;PAS;periodic acid-methanamine silver;osmium removal;etched section;p-toluenesulfonic acid;DMSO

 

数据来源: Taylor

 

摘要:

Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved usingp-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification.

 

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