Staining Etched Epoxy Resin Sections for Light Microscopy
作者:
IwadareTsukasa,
AraiTaizo,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1995)
卷期:
Volume 70,
issue 2
页码: 53-56
ISSN:1052-0295
年代: 1995
DOI:10.3109/10520299509108317
出版商: Taylor&Francis
关键词: azan;2-bromoethylamine;epoxy section;ferrocene;hematoxylin-eosin;kidney biopsy;light microscopy;PAS;periodic acid-methanamine silver;osmium removal;etched section;p-toluenesulfonic acid;DMSO
数据来源: Taylor
摘要:
Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved usingp-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification.
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