Elevated BSC-1 and ROMK Expression in Dahl Salt-Sensitive Rat Kidneys
作者:
Kimberly Hoagland,
Averia Flasch,
Annette Dahly-Vernon,
Elisabete dos Santos,
Mark Knepper,
Richard Roman,
期刊:
Hypertension: Journal of The American Heart Association
(OVID Available online 2004)
卷期:
Volume 43,
issue 4
页码: 860-865
ISSN:0194-911X
年代: 2004
出版商: OVID
关键词: rats, Dahl;Na+, K+-transporting ATPase;sodium;hypertension
数据来源: OVID
摘要:
Abstract—This study compared the expression of enzymes and transport and channel proteins involved in the regulation of sodium reabsorption in the kidney of Dahl salt-sensitive (DS) and salt-resistant Brown-Norway (BN) and consomic rats (SS.BN13), in which chromosome 13 from the BN rat has been introgressed into the DS genetic background. The expression of the Na+/K+/2Cl−(BSC-1) cotransporter, Na+/H+exchanger (NHE3), and Na+-K+-ATPase proteins were similar in the renal cortex of DS, BN, and SS.BN13 rats fed either a low-salt (0.1% NaCl) or a high-salt (8% NaCl) diet. The expression of the BSC-1 and the renal outer medullary K+channel (ROMK) were higher, whereas the expression of the cytochrome P4504A proteins responsible for the formation of 20-hydroxyeicosatetraenoic (20-HETE) was lower in the outer medulla of the kidney of DS than in BN or SS.BN13 rats fed either a low-salt or a high-salt diet. In addition, the renal formation and excretion of 20-HETE was lower in DS than in BN and SS.BN13 rats. These results suggest that overexpression of ROMK and BSC-1 in the thick ascending limb combined with a deficiency in renal formation of 20-HETE may predispose Dahl S rats fed a high-salt diet to Na+retention and hypertension.
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