We investigated the toxicity of Aroclor 1016 to, and uptake by, fry and juvenile and adult sheepshead minnows (Cyprinodon variegatus) in intermittent-flow bioassays lasting 28 days. Survival of eggs, of fry hatched from them, and of juvenile and adult fish apparently was not affected by 0.1, 0.32, 1.0, 3.2, or μg/liter of Aroclor 1016 added to aquaria, but 32 and 100 μg/liter killed newly hatched fry and juvenile and adult fish. Sheepshead minnows accumulated the chemical in proportion to its concentration in the test water. Fry contained 2,500 to 8,100 X the concentration of Aroclor 1016 added to the test water, adults 4,700 to 14,000 X, and juveniles 10,000 to 34,000 X. As much as 77 μg/g of Aroclor 1016 in eggs from exposed adults apparently did not affect survival of embryos and fry.