Bromodeoxyuridine Immunohistochemistry for Evaluating Age-Related Changes in the Rat Mandibular Condyle Decalcified by Intravenous Infusion
作者:
SanoKazuo,
SekineJoji,
PeMichael B.,
InokuchiTsugio,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 5
页码: 297-302
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110038
出版商: Taylor&Francis
关键词: bromodeoxyuridine;temporomandibular joint;S-phase cell;labeling index;decalcification;intravenous infusion
数据来源: Taylor
摘要:
Age-related changes in cell proliferation kinetics of the mandibular condyle were studied in Sprague-Dawley rats using bromodeoxyuridine (BrdUrd) immunohistochemistry in decalcified, paraffin-embedded tissues. Intraperitoneal injection of 40 mg/kg BrdUrd was given 1 hr before animal sacrifice. Continuous perfusion of EDTA solution via the left ventricle shortened the decalcification time. Immunohistochemical staining was performed with monoclonal anti-BrdUrd antibody. BrdUrd labeled cells, i.e., the S-phase cells, were clearly visible with well-preserved cytological detail. Their nuclei exhibited homogeneously stained granules. The labeling index in the intermediate zone of the condyle decreased with increasing age of the animals. This method is useful for evaluating physiological and pathological changes of the rat mandibular condyle as well as other bones and joints.
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