Candid No. 1 Argentine Hemorrhagic Fever Vaccine Protects against Lethal Junin Virus Challenge in Rhesus Macaques
作者:
Kelly T. McKee, Jr.,
Julio G. Barrera Oro,
Anna I. Kuehne,
Joan A. Spisso,
B.G. Mahlandt,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 34,
issue 3
页码: 154-163
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150276
出版商: S. Karger AG
关键词: Junin virus;Candid No. 1;Argentine hemorrhagic fever;Viral hemorrhagic fever;Vaccine;Animal model;Arenavirus
数据来源: Karger
摘要:
The protective efficacy of Candid No. 1, a live-attenuated vaccine against Argentine hemorrhagic fever (AHF), was evaluated in non-human primates. Twenty rhesus macaques immunized 3 months previously with graded doses of Candid No. 1 (16–127, 000 PFU), as well as 4 placebo-inoculated controls, were challenged with 4.41 log10 PFU of virulent P3790 strain Junin virus. All controls developed severe clinical disease; 3 of 4 died. In contrast, all vaccinated animals were fully protected; none developed any signs of AHF during a 105-day follow-up period. Viremia and virus shedding were readily detected in all placebo-vaccinated controls, while virus could be recovered only once (by amplification) from throat swabs of 2 Candid No. 1 vaccinees on day 21. Vigorous secondary-type neutralizing and immunofluorescent antibody responses were seen in most vaccinees that had received 3 logι0 PFU Candid No. 1 or fewer; all others, including those receiving 127,200 PFU, maintained relatively stable titers during follow-up. Candid No. 1 was highly immunogenic and fully protective against lethal Junin virus challenge in rhesus macaques, even at extremely low (16 PFU) vaccine dos
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