The possibilities for direct regeneration in vitro from hippocotyl-cotyledon segments ofGlycyrriza glabra L.grown on different media (MS, DS and WPM) were tested. The maximum regeneration capacity, this means 73.08% explants forming buds and 5.7900.6 the mean number of shoots produced per one explants, was found on DS medium supplemented with 1.0 mg/l BA and 10.0 mg/l adenine. This difference is considerable according to Duncan's multiple range test. The hormone free medium provided a 100% rooting of shoots and 85% survived under in vivo conditions.