The morphology and chaetotaxy of the three larval instars of the genusCalobiusWollaston, 1854 are described and illustrated following the setal pattern ofCalobius quadricollisMulsant, 1844. The study revealed several characters on the cephalic capsule, head appendages, and the thoracic and abdominal segments that can be used to restoreCalobiusas a valid genus. A table showing twenty different features between the larvae of the genusCalobiusand the genusOchthebiusLeach, 1815 is provided.