On the Presence of a Nondopaminergic, Peptidergic Prolactin Release-Inhibiting Factor in Hypothalamic Extracts of Infantile Rats
作者:
Omid Khorram,
Samuel M. McCann,
期刊:
Neuroendocrinology
(Karger Available online 1986)
卷期:
Volume 44,
issue 1
页码: 65-69
ISSN:0028-3835
年代: 1986
DOI:10.1159/000124623
出版商: S. Karger AG
关键词: Prolactin release-inhibiting factor;Dopamine;Prolactin;Pituitary incubation;Spiroperidol;Estradiol;Pronase
数据来源: Karger
摘要:
Crude hypothalamic extracts prepared from brains of 1-day-old rats produced a dose-dependent inhibition of prolactin (Prl) release by adult male hemipituitaries, and to a lesser extent by hemipituitaries of adult ovariectomized (OVX), estrogen-primed rats. These extracts contained 6-fold lower levels of dopamine than adult hypothalami. The inhibitory effect of the adult hypothalamic extracts, contrary to infantile hypothalamic extracts could be blocked by spiroperidol. Digestion of the infantile hypothalamic extracts with pronase totally abolished their Prl release-inhibiting activity, indicating the peptidic nature of this inhibitory substance. In contrast to their effect on Prl release by hemipituitaries, infantile hypothalamic extracts stimulated Prl release from dispersed anterior pituitary cells of OVX estrogen-primed rats, pointing to the importance of estrogen in modulating prolactin release-inhibiting factor (PIF) activity and the possibility that the PIF receptor is trypsin-sensitive.
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